Automated NGS Library Preparation Workstation

Auto-NGS 200

It is a automated NGS library preparation workstation that meets the needs of library preparation, quantitation, multi-targeting and hybrid capture all in one go. The instrument has a built-in 24-well fluorometer and PCR amplification module, which really realizes the convenient operation of sample-to-library, without manual intervention in the whole process.
The 27 plate positions, together with a gripper and a 24-channel pipettor, can realize the relatively simple library preparation of 96 samples, as well as the simplified type of fully automated library preparation and hybrid capture of 16 samples.

INQUIRY

Built-in fluorometer block, automates the whole process
Open platform, suitable for most of the library preparation cartridges on the market
Clear software logic, easy to use, low learning costs

Technical Specifications

Ordering Information

Overview

Key Configuration

Plate Layout

Pipettor

24-Channel Pipettor

Pipetting range 1~200 μL, can be used as 24 channels, 16 channels, 8 channels, single channel or any channel, meeting the needs for reagent dispensing and simultaneous pipetting of multiple samples within the same process.
Suitable for both 50 μL and 200 μL pipette tips, with a CV≤6% for 1 μL pipetting, meeting the needs of high-precision NGS experimental workflows.

Pipetting range	1~200 μL
Pipetting precision	1 μL: ≤8%	2 μL: ≤5%	5 μL: ≤4%	20 μL: ≤1%	100 μL: ≤1%	200 μL: ≤1%
Pipetting accuracy	1 μL: ±10%	2 μL: ±8%	5 μL: ±4%	20 μL: ±2%	100 μL: ±1%	200 μL: ±1%

Gripper

Standard Mechanical Gripper

Can realize pipetting from heating and mixing module to magnet, significantly reducing liquid loss compared to pipettor.
With plate stacking function, new and waste plates are uniformly managed within the same experimental workflow, reducing manual intervention.

Fluorometer

The built-in fluorometer quantitative method is sensitive and highly accurate, making it a reliable dsDNA measurement method.

Automation-specific module can test 24 samples simultaneously.

Accurate quantitation and high accuracy with only 2~20 μL samples.

Lowest detection limit down to 0.02 ng/μL (dsDNA).

Cooperate with the automatic calculation of the software and high-precision pipetting to quickly achieve accurate sampling.

PCR Module

Built in PCR block is safe and reliable,with extremely low cross-contamination rate.

Module temperature control range: 4 °C ~ 99 °C, the temperature range of the thermo lid is 30 °C~120 °C

Temperature precision: ≤0.2 °C @55 °C, temperature accuracy ≤±0.3 °C @55 °C

Temperature uniformity: ≤0.7 °C (@55 °C, 72 °C)

Thermo Shaker Incubator Module

A thermo shaker incubator applied to Allsheng automation workstation. The incubator can be used for heating, incubation and mixing of deepwell plates. The high-precision zero positioning function ensures the safe pipetting by the pipettor, with the anti-vibration technology and 2D motion control.

Temperature range: 30 °C~105 °C

Amplitude: 2 mm, max speed: 3000 rpm

Graphic Design with Clear Logic

NGS series software adopts an open design and can be freely edited and adjusted according to different cartridges. Adopting a graphical interface design, it can be mastered through simple learning.

1

Simple Reagent Input

Free selection of well positions, and support for pre-filled reagents
2

Clear Software Logic

The protocol running framework is completed through drag and drop operations
3

Graphical Step Editing

Easy to get started, with built-in multiple liquid parameters matching precise pipetting requirements
4

Detailed Operation Guidance

Follow the software prompts to place consumables in order to run the protocol

Pathogen Targeted High-Throughput Sequencing

Infectious diseases have always been a major disease threatening human health and life in the world today

At present, the global incidence rate of infectious diseases is rising, and the pathogens are diversified and complex. Early, rapid and accurate identification of pathogenic microorganisms is the prerequisite for effective prevention and treatment of infectious diseases.
Pathogen targeted high-throughput sequencing (tNGS), combined with high-multiplex PCR amplification and high-throughput sequencing technology, can simultaneously detect dozens to hundreds of known pathogenic microorganisms in samples, as well as detect the virulence and resistance genes of pathogenic microorganisms.
For the detection of low concentrations of pathogenic microorganisms, especially their virulence and drug resistance genes, tNGS has the advantage of higher sensitivity and lower cost of detection, compared with pathogenic metagenomic next generation sequencing (mNGS). It also takes into account the DNA and RNA processes, and has advantages in excluding host nucleic acid interference and detecting low concentrations of pathogenic microorganisms.

Model	ASmart-Lib 100	Auto-NGS 100RF	Auto-NGS 180S	Auto-NGS 200
Throughput	24	24	48	96
Pipettor	24-channel	8-channel	24-channel	24-channel
Pipetting range	1~200 μL	1~200 μL	1~200 μL	1~200 μL
Heating shaker module	√	-	√	√
Quality control module	√	√	√	√
PCR module	√	√	√	√
Dimension (W×D×H)	540×670×830 mm	800×774×775 mm	980×793×830 mm	1420×790×800 mm

Note: "Throughput" refers to the instrument's maximum throughput

Model	Auto-NGS 200
Throughput	1~96
Tiles number	27
Pipettor	Channel number: 24-channel (can be used as a single channel)
Pipettor	Pipetting range: 1~200 μL
Pipettor	Pipetting precision (CV): 1 μL≤8 %; 2 μL≤5 %; 5 μL≤4 %; 20 μL≤1 %; 100 μL≤1 %; 200 μL≤1 %
Pipettor	Pipetting accuracy (A): 1 μL≤±10 %; 2 μL≤±8 %; 5 μL≤±4 %; 20 μL≤±2 %; 100 μL≤±1 %; 200 μL≤±1 %
Temperature control module	Module number: 2
Temperature control module	Temperature control range: 4 °C~105 °C
Temperature control module	Accuracy: ≤1 °C
Temperature control module	Uniformity: ≤1 °C @55 °C
Thermal cycler	Temperature control range: thermo lid: 30 °C~120 °C; module: 4 °C~99 °C
Thermal cycler	Uniformity: ≤0.5 °C
Thermal cycler	Accuracy: ≤±0.3 °C
Fluorescence detection throughput	24
Fluorescence detection range	0.4 ng~100 ng
Heating shaker module	Yes
Magnetic module	96-well annular magnetic module
Gripper	Yes
Consumable stacking mode	Adjustable height stack plate module
UV sterilization	Equipped with UV sterilization lamp, HEPA device
Instrument port	USB port
Information management	External scanner for entering sample / reagent information Expandable LIS system connection Expandable cloud database connection
Operating environment	Temperature requirement: 20±5 °C Relative humidity: ≤80%
Power supply	100~240 V, 50/60 Hz, rated power 1200 W
Dimension (W×D×H) mm	1420×790×840 mm
Weight	300 kg

Code	Product description
AS-27040-00	Auto-NGS 200 automated NGS library preparation workstation
AS-27041-01	TIP off box (recommended to store less than 1000 pcs)
AS-27041-02	Reagent rack (place 3×120 mL reservoir)
AS-27041-03	Magnetic plate for workstation (fixed, 96 PCR plate)
AS-27021-02	Standard tray (standard height plate for SBS consumables, such as ALLSHENG boxed TIPs)
AS-27021-03	Heightened tray (higher than the standard tray for SBS consumables, such as ALLSHENG boxed TIPs)
AS-27021-04	PCR tube holder (8-strip PCR tube, non-skirted 96-well PCR plate)
AS-23010-00	Au-Cooler dry bath incubator for workstation (heating and cooling), 24 V DC, 160 W, with CAN
AS-23160-00	Au-Shaker 3020TL automatic locking thermo shaker incubator for workstation (heating), 24 V DC, 120 W, with CAN