Overview
UV / Vis Absorbance
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Wavelength selection is done by using an advanced monochromator system. Any wavelength between 200 to 1000 nm can be selected. Using the spectral scanning feature, the whole spectrum of a sample be scanned in 1 nm increments to allow identification of the optimal measurement wavelength for a new assay. Long life xenon lamp which can be used for 10⁹ times. Fast reading mode only need 15 s for continuous spectral scanning in 1 nm increments between 200~1000 nm. Can be used for spectral scanning, endpoint and kinetic detection.
Fluorescence
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Instrument is equipped with filter-based fluorescence optics and dichroic mirrors for screening applications. Standard applications such as fluorescence-based DNA / RNA quantification assays are not only supported in microplates, but also in low volume u-Nano ultra-micro plate.The independent removable filter modules make it more convenient for users to replace the filter. The filter-based fluorescence optics detection ensures high sensitivity, greater light transmission, precise control over transmitted peak shape, excellent blockage of undesired wavelengths. This is ideal for excitation and emission applications. The filters are also the technically preferred and most cost efficient technology for non-absorbance based assays.
Luminescence
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Feyond series luminescence microplate readers show excellent sensitivity and wide dynamic range in both glow and flash based assays. The PMT enhances the maximum sensitivity of weak luminescence signals, prevents oversaturation of high signals, effectively improves the detection range of luminescence. The optimized light path minimizes signal crosstalk between wells and ensures the accuracy of experimental results. The precise dual-channel injector ensures assay performance even when assaying high-density 384-well plates.
Multiple Shaking Modes
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There are three types of shaking modes: linear, circular, double circular, and a variety of shaking speeds (rpm) can be freely selected, which is more conducive to the realization of kinetic background vibration of different types of samples.
Well Scanning Function
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The scanning detection method of up to 900 points per well is realized by using flexible orbital motion and accurate detection site, which reduces the difference reading caused by different positions.
Key Configuration
u-Nano Ultra-Micro Plate
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Quickly complete high-throughput quantification of nucleic acid and proteins without samples dilution.Independent lower computer software, can quickly read the sample concentration and purity report.16 / 96 samples can be detected at the same time, only 2~4 μL sample volume is needed.
ABS Optical Performance Validation Plate
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ABS optical performance validation plate is mainly used to comprehensively evaluate the performance of absorbance function. It can conveniently, quickly and easily check whether absorbance function of the instrument is working properly. It is suitable for the system check of installation and operation qualification.
Modular Filter
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The easy-to-disassemble modular filter will bring an economical and highly sensitive solution to your fluorescence detections. Only by scanning the QR codes on the module, the instrument can read the filter information to ensure accurate experimental parameters for convenient and quick operation.
MSS-2 Automatic Injector Module
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Equipped with dual automatic injector modules of the instrument is critically important for a myriad of assays, most notably flash luminescence and Ca²⁺ flux assays etc. The instrument is equipped with a standard injector module, which can meet the precise sample addition operation of 384-well plates and realize the possibility of rapid detection of high-throughput plates.The automatic injector module can be purchased separated with the device and can also be upgraded later.Liquid injection protection: The software has a liquid injection protection function to effectively prevent the risk of sample overflow.
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1User Authority ClassificationUser permissions are divided into four levels, with clear permissions. The users have independent accounts and passwords to ensure the safety and confidentiality of experimental results.
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2Intuitive Interface DisplayIntuitive selection of function modes, easy parameter setting. Programs and results are stored independently, making it easy to be found the required applications.
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3Powerful Data Analysis and ProcessProvide multiple data processing methods including blank subtraction, standard curve creation, qualitative analysis, quality control, kinetics and spectral analysis. Algorithm customization: according to your assays needs, can customize the required algorithm.
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4Liberalized User CommunicationThe software has with a shared library, which can store the program, results and standard curves for sharing them with others. The program and standard curve can be created to a QR code, and the required content can be imported only by scanning the code with the instrument.
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Model | |||||||
ABS | ● | ● | ● | ● | ● | ||
FL | ● | ● | ● | ● | ● | ||
LUM | ● | ● | ● | ● | ● | ||
TRF | ● | ● | |||||
FP | ● | ||||||
Incubation | ● | ● | ● | ● | ● | ● | ● |
| Model | Feyond-A300 |
| ABS | |
| Plate | 6-well, 12-well, 24-well, 48-well, 96-well, 384-well, 96 / 384 PCR plate, uNano 16 / 96 ultra-micro plate |
| Reading time (spectrum) | 200~1000 nm <15s (1nm step) |
| Light source | Xenon lamp |
| Wavelength accuracy | 2 nm |
| Wavelength repeatability (SD) | 0.2 nm |
| Half width (FWHM) | <2.5 nm |
| Detector | PD×2 |
| Wavelength range | 200~1000 nm, 1 nm step |
| Measuring range | 0-4 OD |
| Resolution | 0.0001 OD |
| Accuracy @450 nm | Precision mode: ±(1.0%+0.003OD) @ (0.0-2.0OD]; ±2%>@(2.0-3.0OD] |
| Repeatability @450 nm | CV<1.0% or SD<0.003 fast (0.0-3.0OD]; CV<0.5% or SD<0.003 accurate (0.0-3.0OD] |
| Stray light | 0.1 % @220 nm |
| Linear @450 nm | R²≥0.999 @ [0.0 - 3.0 OD] |
| Reading time | 96-well plate: fast <15 s (A1 to H1) |
| FL | |
| Reading mode | Top reading |
| Excitation light source | Xenon lamp |
| Detector | PMT |
| Wavelength range | EX: 200~1000 nm; EM: 270~850 nm |
| Filter EX / EM | EX485 / EM530, EX523 / EM564, EX624 / EM692 (other wavelengths can be replaced) |
| Linear dynamic range | ≥6 logs |
| Detection limit | 1 pM (optimization condition) |
| LUM | |
| Detector | PMT |
| Detection limit | ≤15 amol/well @96-well white plate with flash ATP |
| Linear dynamic range | ≥6 logs |
| Crosstak | ≤0.005% |
| Wavelength range | 200-850 nm |
| Filter | LUM |
| Software | |
| Analysis software | ReaderIt-Ⅱ software |
| Basic Parameter | |
| Read type | Endpoint, kinetics, spectral scanning, and well scanning |
| Plate | 6-well, 12-well, 24-well, 48-well, 96-well, 384-well, 96 / 384 PCR plate, uNano 16 / 96 ultra-micro plate |
| Accessories | u-Nano16 plate, u-Nano96 plate, auto-injector |
| Shaking mode | Linear, circular, double circular (strength and speed adjustable) |
| Incubation temperature | RT. +4 °C~45 °C |
| Temperature accuracy | ±0.5 °C @ 37 °C |
| Software interface | Chinese / English |
| Screen size | 10-inch |
| Operation method | Capacitive screen touch, mouse |
| Data capacity | 10 GB |
| Compatibility | Support PC software, Win 10 and above, 64 bit |
| Network transmission | The test data report can be uploaded to the PC server through FTP |
| Instrument port | 2 USB A ports, 1 USB B port, 1 Ethernet port, Rs232 bus interface (connected to the injector) |
| Power supply | AC 100-240 V, 50-60 Hz |
| Dimension (W×D×H) | 420×550×386 mm |
| Weight | 33 kg |
| Accessory Parameter | |
| u-Nano | |
| Sample number | 1~96 |
| Sample detection volume | 2-4 μL |
| Automatic Injector | |
| Support plate | 6-384 well plate |
| Quantity | 1 / 2 |
| Dispensing volume | 5-1000 μL, 1 μL increment |
| Liquid injection speed | 125-500 μL/s |
| Accuracy | ±1 μL @ 5-50 μL; ±2% @ 51-1000 μL |
| Waste liquid collection | 25 mL |
| Code | Product description |
| AS-19050-00 | Feyond-A300 microplate reader (multi-mode) |
| AS-19051-01 | 485nm-530nm fluorescence filter (standard) |
| AS-19051-02 | 523nm-564nm fluorescence filter (standard) |
| AS-19051-03 | 624nm-692nm fluorescence filter (standard) |
| AS-19051-04 | LUM filter |
| AS-19011-01 | ReaderIt-Ⅱ PC analysis software |
| AS-19011-02 | u-Nano16 ultra-micro plate |
| AS-19011-03 | ABS optical performance validation plate |
| AS-19011-04 | MSS-2 automatic injector |
| AS-19011-05 | u-Nano96 ultra-micro plate |
| RS-CL0101-100T | ATP bioluminescence assay kit (100T) |
| RS-CL0101-500T | ATP bioluminescence assay kit (500T) |
| RS-CL0102-100T | Single luciferase reporter gene test kit (100T) |
| RS-CL0102-500T | Single luciferase reporter gene test kit (500T) |
Product Information
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