Overview

UV / Vis Absorbance
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Wavelength selection is done by using an advanced monochromator system. Any wavelength between 200 to 1000 nm can be selected. Using the spectral scanning feature, the whole spectrum of a sample be scanned in 1 nm increments to allow identification of the optimal measurement wavelength for a new assay. Long life xenon lamp which can be used for 10⁹ times. Fast reading mode only need 15 s for continuous spectral scanning in 1 nm increments between 200~1000 nm. Can be used for spectral scanning, endpoint and kinetic detection.
Fluorescence
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Instrument is equipped with filter-based fluorescence optics and dichroic mirrors for screening applications such as fluorescence polarization, and TR-FRET. Standard applications such as fluorescence-based DNA / RNA quantification assays are not only supported in microplates, but also in low volume u-Nano ultra-micro plate.
The independent removable filter modules make it more convenient for users to replace the filter. The filter-based fluorescence optics detection ensures high sensitivity, greater light transmission, precise control over transmitted peak shape, excellent blockage of undesired wavelengths. This is ideal for excitation and emission applications. The filters are also the technically preferred and most cost efficient technology for non-absorbance based assays.
Luminescence
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Feyond series luminescence microplate readers show excellent sensitivity and wide dynamic range in both glow and flash based assays. The PMT enhances the maximum sensitivity of weak luminescence signals, prevents oversaturation of high signals, effectively improves the detection range of luminescence. The optimized light path minimizes signal crosstalk between wells and ensures the accuracy of experimental results. The precise dual-channel injector ensures assay performance even when assaying high-density 384-well plates.
Time-Resolved Fluorescence (TRF)
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Time-resolved fluorescence is based on lanthanide elements as dyes. When excited, the emission time is much longer than that of ordinary fluorescein. After the excitation light is turned off, the emitted light can still be continuously expressed and released, thereby eliminating the interference of excitation light and stray light.
Time-resolved fluorescence has high sensitivity, strong specificity, good stability, and short operation flow. It is suitable for trace analysis in biology and medicine, hormone detection, viral hepatitis marker detection, target cell marker detection, and drug screening.
Fluorescence Polarization (FP)
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The optimized filter design of Feyond-A500 can effectively reduce detection deviation and is often used to detect intermolecular interactions, such as the determination of drugs and hormones, tyrosine kinase detection, receptor / ligand research, protein / polypeptide interactions, DNA / protein interactions, etc.
Multiple Shaking Modes
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There are three types of shaking modes: linear, circular, double circular, and a variety of shaking speeds (rpm) can be freely selected, which is more conducive to the realization of kinetic background vibration of different types of samples.
Well Scanning Function
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The scanning detection method of up to 900 points per well is realized by using flexible orbital motion and accurate detection site, which reduces the difference reading caused by different positions.

Key Configuration

u-Nano Ultra-Micro Plate
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Quickly complete high-throughput quantification of nucleic acid and proteins without samples dilution.
Independent lower computer software, can quickly read the sample concentration and purity report.
16 / 96 samples can be detected at the same time, only 2~4 μL sample volume is needed.
ABS Optical Performance Validation Plate
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ABS optical performance validation plate is mainly used to comprehensively evaluate the performance of absorbance function. It can conveniently, quickly and easily check whether absorbance function of the instrument is working properly. It is suitable for the system check of installation and operation qualification.
Modular Filter
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The easy-to-disassemble modular filter will bring an economical and highly sensitive solution to your fluorescence detections. Only by scanning the QR codes on the module, the instrument can read the filter information to ensure accurate experimental parameters for convenient and quick operation.
MSS-2 Automatic Injector Module
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Equipped with dual automatic injector modules of the instrument is critically important for a myriad of assays, most notably flash luminescence and Ca²⁺ flux assays etc. The instrument is equipped with a standard injector module, which can meet the precise sample addition operation of 384-well plates and realize the possibility of rapid detection of high-throughput plates.
The automatic injector module can be purchased separated with the device and can also be upgraded later.
Liquid injection protection: The software has a liquid injection protection function to effectively prevent the risk of sample overflow.
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User Authority Classification Intuitive Interface Display Powerful Data Analysis and Process Liberalized User Communication
  • 1
    User Authority Classification
    User permissions are divided into four levels, with clear permissions. The users have independent accounts and passwords to ensure the safety and confidentiality of experimental results.
  • 2
    Intuitive Interface Display
    Intuitive selection of function modes, easy parameter setting. Programs and results are stored independently, making it easy to be found the required applications.
  • 3
    Powerful Data Analysis and Process
    Provide multiple data processing methods including blank subtraction, standard curve creation, qualitative analysis, quality control, kinetics and spectral analysis. Algorithm customization: according to your assays needs, can customize the required algorithm.
  • 4
    Liberalized User Communication
    The software has with a shared library, which can store the program, results and standard curves for sharing them with others. The program and standard curve can be created to a QR code, and the required content can be imported only by scanning the code with the instrument.
Model
ABS


FL


LUM


TRF





FP






Incubation


ModelFeyond-A500
ABS
Plate6-well, 12-well, 24-well, 48-well, 96-well, 384-well, 96 / 384 PCR plate, uNano 16 / 96 ultra-micro plate
Reading time (spectrum)200~1000 nm <15s (1nm step)
Light sourceXenon lamp
Wavelength accuracy2 nm
Wavelength repeatability (SD)0.2 nm
Half width (FWHM)<2.5 nm
DetectorPD×2
Wavelength range200~1000 nm, 1 nm step
Measuring range0-4 OD
Resolution0.0001 OD
Accuracy @450 nmPrecision mode: ±(1.0%+0.003OD) @ (0.0-2.0OD]; ±2%>@(2.0-3.0OD]
Repeatability @450 nmCV<1.0% or SD<0.003 fast (0.0-3.0OD]; CV<0.5% or SD<0.003 accurate (0.0-3.0OD]
Stray light0.1 % @220 nm
Linear @450 nmR²≥0.999 @ [0.0 - 3.0 OD]
Reading time96-well plate: fast <15 s (A1 to H1)
FL
Reading modeTop reading
Excitation light sourceXenon lamp
DetectorPMT
Wavelength range
EX: 200~1000 nm; EM: 270~850 nm
Filter EX / EMEX485 / EM530, EX523 / EM564, EX624 / EM692 (other wavelengths can be replaced)
Linear dynamic range≥6 logs
Detection limit1 pM (optimization condition)
LUM
DetectorPMT
Detection limit≤15 amol/well @96-well white plate with flash ATP
Linear dynamic range≥6 logs
Crosstak≤0.005%
Wavelength range200-850 nm
FilterLUM
TRF
Filter EX / EM1 group: EX365 / EM612
Detection limit0.02pM (optimization condition)
FP
Filter EX / EM2 groups: EX485 / EM530
Detection limitsd ≤ 5 mP @1 nm fluorescein sodium
Software
Analysis softwareReaderIt-Ⅱ software
Basic Parameter
Read typeEndpoint, kinetics, spectral scanning, and well scanning
Plate6-well, 12-well, 24-well, 48-well, 96-well, 384-well, 96 / 384 PCR plate, uNano 16 / 96 ultra-micro plate
Accessoriesu-Nano16 plate, u-Nano96 plate, auto-injector
Shaking modeLinear, circular, double circular (strength and speed adjustable)
Incubation temperatureRT. +4 °C~45 °C
Temperature accuracy±0.5 °C @ 37 °C
Software interfaceChinese / English
Screen size10-inch
Operation methodCapacitive screen touch, mouse
Data capacity10 GB
CompatibilitySupport PC software, Win 10 and above, 64 bit
Network transmissionThe test data report can be uploaded to the PC server through FTP
Instrument port2 USB A ports, 1 USB B port, 1 Ethernet port, Rs232 bus interface (connected to the injector)
Power supplyAC 100-240 V, 50-60 Hz
Dimension (W×D×H)420×550×386 mm
Weight33 kg
Accessory Parameter
u-Nano
Sample number1~96
Sample detection volume2-4 μL
Automatic Injector
Support plate6-384 well plate
Quantity1 / 2
Dispensing volume5-1000 μL, 1 μL increment
Liquid injection speed125-500 μL/s
Accuracy±1 μL @ 5-50 μL; ±2% @ 51-1000 μL
Waste liquid collection25 mL


CodeProduct description
AS-19070-00Feyond-A500 microplate reader (multi-mode)
AS-19051-01485nm-530nm fluorescence filter (standard)
AS-19051-02523nm-564nm fluorescence filter (standard)
AS-19051-03624nm-692nm fluorescence filter (standard)
AS-19051-04LUM filter
AS-19051-05365nm-612nm time-resolved fluorescence filter (standard)
AS-19051-06485nm-530nm fluorescence polaroid filter (standard)
AS-19011-01ReaderIt-Ⅱ PC analysis software
AS-19011-02u-Nano16 ultra-micro plate
AS-19011-03ABS optical performance validation plate
AS-19011-04MSS-2 automatic injector
AS-19011-05u-Nano96 ultra-micro plate
RS-CL0101-100TATP bioluminescence assay kit (100T)
RS-CL0101-500TATP bioluminescence assay kit (500T)
RS-CL0102-100TSingle luciferase reporter gene test kit (100T)
RS-CL0102-500TSingle luciferase reporter gene test kit (500T)


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