Solution|Hangzhou Allsheng Instruments

CRC Early Screening Sample Preparation

Colorectal cancer (CRC) is the third most common cancer worldwide and the second leading cause of cancer-related deaths. Most cases of colorectal cancer originate from tumor precursor pathology and last for 10~15 years. The main tumor screening techniques now commonly used are medical imaging and tissue biopsy, in addition to tumor marker testing. The traditional early cancer screening is not suitable because of the high reliance on physicians and the lack of obvious early pathology.

With the rapid development of molecular biology and its increasingly widespread clinical application, liquid biopsy has been developed, and among various new detection methods, liquid biopsy is considered to be the most promising early screening technique. ctDNA extraction is relatively simple and therefore widely used, due to its concealment in the early stages of colorectal cancer, the patient's compliance with the examination and the convenience of the detection methods. Early stage tumor cells secrete ctDNA into the blood or feces, and the extraction of these samples and the use of certain detection methods can detect colorectal cancer signals at an early stage. Currently, these signals are differentiated by gene mutations, gene methylation levels, and abnormal copy values as criteria.

In view of such characteristics, Allsheng offers both semi-automatic and fully automatic solutions from cfDNA extraction to DNA methylation conversion and purification, to meet the needs of different volume applications.

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CRC Early Screening Sample Preparation

Nucleic Acid Extraction

When the samples are feces, the samples often need to go through centrifugation, supernatant extraction, and external lysis. The overall reaction system of the subsequent sample is less than 3 mL, and Auto-Pure 32A/48/96 nucleic acid purification system can meet the application requirements. Users can select appropriate models for extraction based on their actual needs such as reaction system and single maximum sample size.
When the sample is plasma, the concentration of cfDNA in plasma is often low, so the extraction process often requires a large volume of sample. The maximum reaction systems of Auto-Pure 20B/24D are 5 mL and 10 mL respectively, and the minimum elution volume is 50 μL. While increasing the sample loading amount, it ensures sufficient small volume elution, enabling the extraction of cfDNA in plasma samples. The maximum sample throughput for 20B and 24D are 20 and 24. Due to the special consumables design, both instruments can flexibly match tube strips based on the actual number of samples.

Instrument Features

Auto-Pure 32A/48/96 Nucleic Acid Purification System
32A/96: 1 mL reaction system  48: 3 mL reaction system; sample throughput 32/48/96

Auto-Pure 20B/24D Nucleic Acid Purification System
10 mL reaction system, sample throughput 1~24, 50 μL elution

Experimental Data

Concentration Measurement

When the samples are feces, due to the relatively high concentration of nucleic acids in feces, the Nano series micro-spectrophotometer can be used to measure the sample concentration after the extraction is completed to quickly assess the effect of the previous sample extraction and provide data for subsequent homogenization.
When early screening sample is blood plasma, or when blood plasma is applied for companion diagnostic samples of colorectal cancer, the concentration of nucleic acid is often low, and the accuracy of concentration results is low when the concentration detected by micro-spectrophotometer is lower than 10 ng/μL, so Fluo series fluorometer can be chosen as a concentration measurement tool.

Instrument Features

Nano Series Micro-spectrophotometer:
1~2 μL sample loading, easy to use, multiple data output methods

Fluo Series Fluorometer:
1~20 μL sample loading, Max. 8 sample throughput, lowest detection concentration 0.1pg/ μL

Experimental Data

Methylation Conversion

MSC-3000 thermo shaker incubator can realize the demand for variable Temp. incubation of samples. The Temp. control range is 0 °C~105 °C, covering the Temp. range involved in methylation conversion. The instrument can support up to 5 Temp. points of automatic Temp. change to meet the needs of the transformation process of methylation conversion kits that require multiple Temp. points of automatic change. The instrument supports the thermo lid, with the thermo lid block, which can effectively prevent the generation of condensation on the tube wall and cap to avoid the concentration change of each component in the tube.

Instrument Features

0 °C~105 °C
Support thermo lid function
Automatic temperature change at 5 temperature points

Experimental Data

Product Purification

Auto-Pure 96 / Auto-Pure 48 nucleic acid purification system uses magnetic bead method to extract nucleic acid, the maximum reaction system is 1 mL and 3 mL, to meet the extraction needs of different kits, the maximum reaction throughput is 96 and 48. Equipped with a large volume nucleic acid extractor, it can realize the purification of 96 / 48 samples after the conversion.

Instrument Features

1 mL / 3 mL Max. reaction system
Sample throughput 1~96 / 1~48
Open design

Experimental Data

Nucleic Acid Extraction
Concentration Measurement
Methylation Conversion
Product Purification