Solution|Hangzhou Allsheng Instruments

Pathogenic Microorganism Detection

Metagenomics is a discipline that "bypasses the isolation and cultivation of individual microorganisms and applies genomics research strategies to study the genetic composition and community functions of microorganisms in natural environment / clinical samples".

Metagenomics next-generation sequencing (mNGS) is a technique to obtain nucleic acid sequences in samples by rapid sequencing with a next-generation sequencing platform, and further compare them with the genome sequences of individual species to know the species and proportion of microorganisms in the samples, so it has been widely used in clinical microbiology detection in recent years.

In addition to macrogenomic detection, droplet digital PCR (ddPCR) has been used to determine the relative and/or absolute abundance of pathogenic microorganisms, based on microfluidic technology that separates amplification reactions into droplets of >20,000 individuals, allowing the absolute quantification of DNA from up to 4 target organisms or genes simultaneously, with a detection limit of 10^(-5) relative abundance.

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Pathogenic Microorganism Detection

Sample Crushing and Grinding

Since mNGS samples are often a mixture of multiple organisms, many samples are difficult to lyse, such as mycobacterium tuberculosis and cryptococcus in respiratory samples. The use of a homogenizer with different grinding media can effectively ensure the detection of difficult-to-lyse pathogens, and the program can be optimized to take into account the integrity of nucleic acids of easy-to-lyse pathogens.

Instrument Features

6*2 mL / 24*2 mL
Direct crushing without processing
Eliminate cross-contamination

Experimental Data

Nucleic Acid Extraction

When the pathogenic microorganism detection method is amplicon sequencing technology or hybridization sequencing technology, the sample often needs to go through the process of PCR amplification, library construction and other processes before it can be used for subsequent detection. The initial sample concentration demand is not very high, Auto-Pure 32A/96 nucleic acid purification system 1 mL reaction system can meet the demand. The open software design can be adapted to different extraction kits. Users can select the appropriate model for extraction according to the actual requirements of the reaction system and the maximum sample volume.

Instrument Features

1 mL reaction system
Sample throughput 32/96
Open design

Experimental Data

Library Preparation

Auto-NGS 100R automated NGS library preparation workstation is designed with 9 standard tiles + (TIP off box + waste liquid area) for construction of 24 sample libraries in one run. The instrument is equipped with built-in magnetic plate, PCR amplification block and other functional blocks to automate the library construction process based on direct sample loading of nucleic acid samples, including fragmentation, end repair, adaptor connection, PCR amplification, library mixing, and etc.

Instrument Features

Built-in PCR amplification block
Construct 24 sample libraries in one run
1~200 μL 8-channel pipettor

Experimental Data

Concentration Measurement

The need for precise quantification of sample concentrations before and after library construction is often limited by the principle of detection and the lower limit of detection, and micro-spectrophotometers are often unable to meet the needs of such samples. Fluorometer with corresponding detection dyes can specifically determine the concentration of the test substance, with the minimum detection concentration of 0.1 pg/μL (dsDNA) for Fluo-200 and 1 pg/μL (dsDNA) for Fluo-800.
In addition to the fluorometer, the fluorescence microplate reader can also be used for low concentration samples, with a maximum sample size of 96, which is particularly suitable for customers with larger sample sizes and reduces operating time.

Instrument Features

Fluo Series Fluorometer:
1-20 μL sample loading, maximum 1/8 sample throughput, minimum detection concentration 0.1 pg/μL

Feyond F100 Fluorescence Microplate Reader:
1-20 μL sample loading, maximum 96 sample throughput, minimum detection concentration 0.5 pg/μL

Experimental Data

Sample Crushing and Grinding
Nucleic Acid Extraction
Library Preparation
Concentration Measurement