Microplate Reader

Feyond-MF280

Feyond-MF280, as a distinctive dual-function microplate detection platform, adopts a unique design of dual optical elements. It features two detection modes: full-wavelength absorbance (UV / Vis / NIR) and fluorescence intensity (FI).

The absorbance detection mode covers a wide wavelength range from ultraviolet to visible light, enabling precise measurement of the degree to which the sample absorbs light at different wavelengths.

The fluorescence detection mode, on the other hand, makes use of the characteristic that fluorescent substances emit fluorescence under the excitation of light at a specific wavelength. It has high sensitivity and specificity, and is suitable for detecting trace amounts of biomolecules, intracellular signal transduction, analysis of enzyme activity, etc., playing an important role in fields such as drug screening and cell biology research.

INQUIRY

Absorbance wavelength range: 190~1000 nm
Support up to 8 excitation and emission filter combinations, easily achieving FRET measurements
Fluorescence detection limit as low as 2 pM

Technical Specifications

Ordering Information

Overview

Function Configuration Overview

Absorbance

Using the monochromator design, it only need 15 s for continuous spectral scanning in 1 nm increments between 190~1000 nm. It supports ultraviolet to near-infrared spectrum analysis, and the wavelength can be freely selected to easily realize various application detection. In addition, the linear correlation coefficient can reach more than 0.999 within 0-3.5OD.

Fluorescence

Utilizing a combination of xenon lamps, filters, and high-sensitivity photomultiplier tube (PMT), this system significantly enhances detection sensitivity. The automated filter wheel design supports up to 8 excitation and emission filter combinations, offering 64 customizable configurations without manual module replacement. It enables simultaneous detection of 8 fluorescence channels per cycle, effortlessly achieving both fluorescence intensity and FRET (Fluorescence Resonance Energy Transfer) measurements.

For multi-channel detection applications, the software has two reading modes, which can be selected to read by well or by plate according to experimental needs.

Multiple Shaking Modes

There are three types of shaking modes: linear, circular, double circular, and a variety of shaking speeds (rpm) can be freely selected, which is more conducive to the realization of kinetic background vibration of different types of samples.

Well Scanning Function

The scanning detection method of up to 900 points per well is realized by using flexible orbital motion and accurate detection site, which reduces the difference reading caused by different positions.

Key Configuration

u-Nano Ultra-Micro Plate

Quickly complete high-throughput quantification of nucleic acid and proteins without samples dilution.

Independent lower computer software, can quickly read the sample concentration and purity report.

16 / 96 samples can be detected at the same time, only 2~4 μL sample volume is needed.

ABS Optical Performance Validation Plate

ABS optical performance validation plate is mainly used to comprehensively evaluate the performance of absorbance function. It can conveniently, quickly and easily check whether absorbance function of the instrument is working properly. It is suitable for the system check of installation and operation qualification.

MSS-2 Automatic Injector Module

Equipped with dual automatic injector modules of the instrument is critically important for a myriad of assays, most notably flash luminescence and Ca²⁺ flux assays etc. The instrument is equipped with a standard injector module, which can meet the precise sample addition operation of 384-well plates and realize the possibility of rapid detection of high-throughput plates.

The automatic injector module can be purchased separated with the device and can also be upgraded later.

Liquid injection protection: The software has a liquid injection protection function to effectively prevent the risk of sample overflow.

1

PC Control Version: User Authority Classification

Three levels, with independent accounts and passwords.
2

Liberated Process

Freely combine detection types and functions according to experimental needs.
3

Built-in Algorithms

Provide multiple data processing methods including blank subtraction, standard curve creation, qualitative analysis, quality control, kinetics and spectral analysis.
4

Open Kinetics

During kinetics detection, the process can be paused and resumed, allowing other detection experiments to be inserted to enhance detection efficiency.
5

Kinetics Protection

During longtime kinetics detections, if the computer occurs an abnormal disconnection, the instrument will automatically save the detection data. After reconnection, the data will be uploaded.
6

3D Scanning

Simultaneous excitation and emission wavelength scanning for direct observation of sample features.

Model	Feyond-MF280 (2-Mode)	Feyond-ML280 (2-Mode)	Feyond-M380 (3-Mode)	Feyond-M480 (4-Mode)	Feyond-M580 (5-Mode)	Feyond-M380_Pro (3-Mode)	Feyond-M480_Pro (4-Mode)	Feyond-M580_Pro (5-Mode)	Feyond-M580_Plus (5-Mode)
ABS	●	●	●	●	●	●	●	●	●
FL	●		●	●	●	●	●	●	●
FRET	●		●	●	●	●	●	●	●
LUM		●	●	●	●	●	●	●	●
BRET						●	●	●
TRF				●	●		●	●	●
TR-FRET							●		●
FP					●			●	●
Incubation	●	●	●	●	●	●	●	●	●

Model	Feyond-MF280
ABS
Plate	6-well, 12-well, 24-well, 48-well, 96-well, 384-well, 96 / 384 PCR plate, 16 / 96 uNano ultra-micro plate
Reading time (spectrum)	190~1000 nm <15s (1 nm step)
Light source	Xenon lamp
Wavelength accuracy	2 nm
Wavelength repeatability (SD)	0.2 nm
Half width (FWHM)	<2.5 nm
Detector	PD×2
Wavelength range	190~1000 nm, 1 nm step
Measure range	0 - 4 OD
Resolution	0.0001 OD
Accuracy @450 nm	Precision mode: ±(1.0%+0.003OD) @(0.0-2.0OD]; ±2%>@(2.0-3.0OD]
Repeatability @450 nm	CV<1.0% or SD<0.003 fast (0.0-3.0OD]; CV<0.5% or SD<0.003 accurate (0.0-3.0OD]
Stray light	0.1% @220 nm
Linear @450 nm	R²≥0.999 @[0.0 - 3.0 OD]
Reading time	96-well plate: fast <15s (A1 to H1)
FL
Reading mode	Top reading
Excitation light source	Xenon lamp
Detector	PMT
Wavelength range	EX: 200~1000 nm; EM: 270~850 nm
Filter EX / EM	3 groups: EX470 / EM525, EX523 / EM564, EX624 / EM692 (other wavelengths can be replaced)
Linear dynamic range	≥6 logs
Detection limit	2 pM (optimization condition)
Software
Analysis software	ReaderIt-S1 software
Basic Parameter
Read type	Endpoint, kinetics, spectral scanning, spectral kinetics, and well scanning
Plate	6-well, 12-well, 24-well, 48-well, 96-well, 384-well, 96 / 384 PCR plate, 16 / 96 uNano ultra-micro plate
Accessories	16 / 96 uNano ultra-micro plate, auto-injector
Shaking mode	Linear, circular, double circular (strength and speed adjustable)
Incubation temperature	RT. +4 °C ~ 45 °C
Temperature accuracy	±0.5 °C @37 °C
Software interface	Chinese / English
Screen size	10-inch
Operation method	PC (Windows system)
Data capacity	10 GB
Compatibility	Support PC software, Win 10 and above, 64 bit
Network transmission	The test data report can be uploaded to the PC server through FTP
Instrument interface	1 USB B port, Rs232 bus interface
Power supply	AC 100-240 V, 50-60 Hz
Dimension (W×D×H)	320×435×305 mm
Weight	25 kg
Accessory Parameter
u-Nano
Sample number	u-Nano 16: 1~16, u-Nano 96: 1~96
Sample detection volume	2~4 μL
Automatic Injector
Support plate	6~384 well plate
Quantity	1 / 2
Dispensing volume	5~1000 μL, 1 μL increment
Liquid injection speed	125~500 μL/s
Accuracy	±1 μL @5~50 μL; ±2% @51~1000 μL
Waste liquid collection	25 mL

Code	Product description
AS-19120-00	Feyond-MF280 microplate reader (without screen)
AS-19111-01	ReaderIt-S1 PC analysis software
AS-19111-02	470nm-525nm fluorescence filter (standard)
AS-19111-03	523nm-564nm fluorescence filter (standard)
AS-19011-02	u-Nano 16 ultra-micro plate
AS-19011-03	ABS optical performance validation plate
AS-19011-04	MSS-2 automatic injector
AS-19011-05	u-Nano 96 ultra-micro plate